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perbb2 y122  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc perbb2 y122
    Perbb2 Y122, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perbb2+y122/Phospho-HER2%2FErbB2+(Tyr1221%2F1222)+Antibody/pmc05338732-253-31-59
    Average 94 stars, based on 106 article reviews
    perbb2 y122 - by Bioz Stars, 2026-11
    94/100 stars

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    other:

    Article Title: High-Level Clonal FGFR Amplification and Response to FGFR Inhibition in a Translational Clinical Trial
    Article Snippet: Antibodies used were phosphorylated (p) AKT Ser473 (4058), pAKT T308 (2965), AKT (4691), p4EBP1 T37/46 (2855), 4EBP1 (9452), pERK1/2-Thr202/Tyr204 (4370), ERK1, 2 (9102), EGFR (2232), pEGFR Y1068 (3777), pERBB3 Y1289 (4791), pERBB2 Y1221/1222 (2249), pFGFR Y653/654 (3471), pFRS2a (3864), pIGF1R (3918), IGF1R (9750), p85 PI3K (4292), PIK3CA (4255), PTEN (9559), phospho-ribosomal protein S6 (5364), ribosomal protein S6 (2217; all Cell Signaling Technology); β-Actin (A5441; Sigma); FGFR2 (sc-122), EGFR (sc-03), PARP1 (sc-8003), and FRS2 (sc-8318; Santa Cruz Biotechnology); ERBB3 (ab32121) and FGFR1 (ab76464; Abcam); Grb2 (610111; BD Transduction Laboratories); p85 PI3K (ABS233; Millipore); and AZD4547 and AZD8055 were provided by AstraZeneca.

    Article Title: Identification of determinants of aggressive prostate cancer
    Article Snippet: ECL substrate (Millipore) was used for detection and development on GE/Amersham film.

    Blocking Assay:

    Article Title: A combinatorial strategy for treating KRAS mutant lung cancer
    Article Snippet: Proteins were separated by SDS-Page and immunoblotted and transferred to polyvinyl difluoride (PVDF) membranes (Millipore) according to standard protocols. .. Membranes were immunoblotted with antibodies against pERK T202/Y204 (9101), tERK (9107), pAKT S473 (4060), tAKT (9272), pFRS2 Y436 (3861), pSTAT3 Y705 (9145), pMEK S217/221 (9154), MEK (4694), pMET Y1234/1235 (3077), MET (8198), pERBB2 Y1221/1222 (2243), pEGFR Y1068 (3777), EGFR (4267), pERBB3 Y1289 (4791), and PTEN (9559) from Cell Signaling; CRAF (SC-227), and BRAF (SC-5284) from Santa Cruz Biotechnology; and KRAS (WH0003845M1) from Sigma in 5% BSA in TBS blocking buffer. .. Phospho-lysis buffer (50 mM Tris pH 7.5, 1% Tween-20, 200 mM NaCl, 0.2% NP-40) supplemented with phosphatase inhibitors (5 mM Sodium Fluoride, 1 mM Sodium Orthovanadate, 1 mM Sodium Pyrophosphate, 1 mM β-glycerophosphate), and protease inhibitors (Protease Inhibitor Cocktail Tablets, Roche) was used for cell lysis and protein concentration was determined by Bradford protein Assay kit (Biorad).



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    94
    Cell Signaling Technology Inc perbb2 y122
    Perbb2 Y122, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perbb2+y122/Phospho-HER2%2FErbB2+(Tyr1221%2F1222)+Antibody/pmc05338732-253-31-59
    Average 94 stars, based on 1 article reviews
    perbb2 y122 - by Bioz Stars, 2026-11
    94/100 stars
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